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Vaccinia virus ts mutant
Vaccinia virus ts mutant
規(guī)格:
貨期:
編號(hào):B243164
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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DNA
RNA

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凍干粉
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產(chǎn)品名稱 Vaccinia virus ts mutant
商品貨號(hào) B243164
Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts47 (Dales isolate 6828)
Applications
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U12.
Positive for Mycoplasma No
Biosafety Level 2 CDC BMBL recommends vaccination.
Isolation
derived from existing strain
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned a Dales EM category of P, characterized by immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U12.
Effect on Host
Yes, Cytopathic effect: Rounding and sloughing of infected cells
CPE: enlargement, plaques with rounding and eventual sloughing
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host: BSC-40 (ATCC? CRL-2761
Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 31.0°C
Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent [not 100% confluent] for inoculation.
Cryopreservation
Storage temperature: -70°C
Name of Depositor S Dales, RC Condit, S Dales
Special Collection ATCC
Source
derived from existing strain
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain.

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Vaccination recommended for laboratory staff manipulating vaccinia, monkeypox, or cowpox viruses [CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4].

or colder

Non-permissive incubation temperature is 39.5C. This mutant was assigned a Dales EM category of P. It is assigned the Condit map location U12. This subtype is distinguishable from the parent IHD-J strain by its ability to elicit polykaryocytosis in all mammalian and avian cells tested and by its inability to induce an active hemagglutinin at the plasma membrane.

or colder

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