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pNK2809 [pTN10LUK]
pNK2809 [pTN10LUK]
規(guī)格:
貨期:
編號:B241696
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pNK2809 [pTN10LUK]
商品貨號 B241696
Designations pNK2809 [pTN10LUK]
Species Escherichia coli (Migula) Castellani and Chalmers
Depositors N Kleckner
Vector
Construct size (kb): 11.30000019073486
Insert
DNA: genomic
Insert lengths(kb): 9.199999809265137
Gene product: ATP phosphoribosyltransferase [URA3]
Insert Size (kb) 9.200
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): HindIII--13.0; PstI--7.5, 5.5; EcoRI--6.4, 4.3, 2.3; BamHI--uncut; XbaI--uncut.
For insertional mutagenesis of bacterial or yeast genes carried in bacteriophage lambda vectors.
Derived from pNK81 by replacing 1.050 kb of uncharacterized DNA and the his promoter with 4 tandem repeats of the rrnB transcriptional terminator.
Contains the mini-Tn10-LUK insert that consists of the following features: IS10R - nt 63 - promoterless lacZ element starting at codon 8 - nt 3140 - URA3 - nt 4320 - kanR - nt 6023 - IS10L - nt 6090.
The order of the major features in this plasmid is: EcoRI - rrnB terminators - Tn10-LUK in hisG - hisD - EcoRI - pMB1 ori - ampR.
Classification Enterobacteriaceae, Escherichia
References

Huisman O, et al. A Tn10-lacZ-kanR-URA3 gene fusion transposon for insertion mutagenesis and fusion analysis of yeast and bacterial genes. Genetics 116: 191-199, 1987. PubMed: 3038670

Kleckner N, et al. Uses of transposons with emphasis on Tn10. Methods Enzymol. 204: 139-180, 1991. PubMed: 1658561

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

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