日本一在线中文字幕heydvd,热这里只有精品久久免费,久久久久久精品齐齐,a级国产乱理伦片在线观看al色戒,成人免费毛片美女,国产精品jizz在线观看,国产一区二区三区欧美精品,激情久久av一区av二区av三区,久久国产精品免费一区二区,久久久久久精品国产欧美

Size (kb): 4.320

Vector: pFN476 (plasmid)

Promoters: Promoter lac

Construction: pGEM3Z, pKA101-6

Marker(s):ampR

Construct size (kb): 4.320

Features: insert detection: lacZ'
marker(s): ampR
other: par
promoter: lac
promoter for expression: T7
promoter for in vitro transcription: SP6
replicon: pSC101
replicon: rep (pSC101)
MCS: pGEM-3Z; HindIII...EcoRI
restriction site: BamHI
coding sequence: tnpR

expression vector T7 promoter

vector permitting RNA synthesis in vitro

vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase

vector with low copy number

Restriction digests of the clone give the following sizes (kb): EcoRI--4.2; HindIII--4.2; PstI--3.1, 1.1.

Low copy number vector to express transcripts in vivo from the T7 promoter and to minimize toxic gene effects.

Plasmid contains the pSC101 replicon and is maintained at 1-5 copies per cell.

Low copy number minimizes the effect of uninduced expression of T7 RNA polymerase, reducing transcription of potentially toxic inserts.

T7 RNA polymerase can also be provided by infection of F' hosts with M13mGP1-2 (ATCC 40303).

Temperature: 37.0°C