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Size (kb): 13.75

Vector: pMMB33 (cosmid)

Construction: RSF1010, pHC79

Marker(s):kanR

Construct size (kb): 13.75

Features: marker(s): kanR
replicon: R300B

shuttle vector

vector for constructing genomic libraries

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

A broad host range cosmid vector.

It was constructed by replacing the 0.75 kb PstI fragment of pMMB7 by the 5.7 kb PstI fragment of pMMB31, which contains the lambda-cos site and the Km-resistance gene. It differs from pMMB34, ATCC# 37249, only by the orientation of the 5.7 kb fragment.

Temperature: 30.0°C